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UID:20250307T2141Z-1741383679.6519-EO-32444-1@10.73.10.235
STATUS:CONFIRMED
DTSTAMP:20260713T181014Z
CREATED:20250307T213532Z
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DTSTART;TZID=America/Chicago:20250320T160000
DTEND;TZID=America/Chicago:20250320T170000
SUMMARY: Biophysical Evening with Dr. Matthew Lew (WashU)
DESCRIPTION: Department of Biochemistry & Molecular Biophysics Washington U
 niversity Biochemistry/Biophysics (WUBB) Community BIOPHYSICAL EVENING “Sin
 gle-Molecule Orientation-Localization Microscopy: New Challenges and Biophy
 sical Insights” Abstract: Single-molecule orientation-localization microsco
 py builds upon photoswitching mechanisms used in localization microscopy (S
 MLM) to measure both the positions and orientations of fluorophores with na
 noscale resolution. This 6D information enables biophysicists to visualize 
 both the […]
X-ALT-DESC;FMTTYPE=text/html: <p>Department of Biochemistry & Molecular Bio
 physics<br />Washington University Biochemistry/Biophysics (WUBB) Community
 <br /><strong>BIOPHYSICAL EVENING</strong></p><h4><strong><em>“Single-Molec
 ule Orientation-Localization Microscopy: New Challenges and Biophysical Ins
 ights”</em></strong></h4><p><strong><em>Abstract</em></strong><em>: Single-
 molecule orientation-localization microscopy builds upon photoswitching mec
 hanisms used in localization microscopy (SMLM) to measure both the position
 s and orientations of fluorophores with nanoscale resolution. This 6D infor
 mation enables biophysicists to visualize both the morphology and organizat
 ion of biomolecular assemblies simultaneously. We have recently found that 
 it is impossible for polarization-sensitive imaging to distinguish a single
  rotating (“wobbling”) molecule from a pair of molecules that spatially coi
 ncide\, but measuring a fluorophore’s absorption and emission dipole moment
 s simultaneously offers a solution to this challenge. I’ll also review our 
 group’s recent use of fluorogenic probes to visualize the dynamic architect
 ures of amyloid aggregates and biomolecular condensates.</em></p><p><strong
 >Matthew Lew\, PhD.<br /></strong>Associate Professor of Electrical & Syste
 ms Engineering<br />Washington University in Saint Louis</p><p><strong>Date
 </strong>: Thurs\, March 20th @ 4:00 p.m.<br /><strong>Location</strong>: C
 onnor Auditorium\, Farrell Learning & Teaching Center\, 1<sup>st</sup> Floo
 r<br />Reception to follow in 264 McDonnell Sciences</p>
CATEGORIES:Seminars / Lectures
LOCATION:Connor Auditorium
GEO:38.635402;-90.262703
ORGANIZER;CN="Eric":MAILTO:e.eggers@wustl.edu
URL;VALUE=URI:https://calendar.med.wustl.edu/calendar/event/biophysical-eve
 ning-with-dr-matthew-lew-washu/
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